Unexpected Multiple Bands After Plasmid Linearization: Insights on Gel Electrophoresis Results - видео

Here are some recommendations to help avoid these issues: Optimize Enzyme Conditions: Use a fresh batch of restriction enzyme and ensure its stored properly to maintain activity. Double-check the incubation time and temperature specified for the enzyme. Insufficient time or incorrect temperature can lead to incomplete digestion. Consider using an excess of enzyme e.g. 510 units per µg of DNA to ensure complete digestion. Use High-Quality Purified Plasmid: Ensure the plasmid DNA is highly pure and...
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